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Figure 1 | BMC Psychiatry

Figure 1

From: Monitoring candidate gene expression variations before, during and after a first major depressive episode in a 51-year-old man

Figure 1

RT-qPCR analysis of SLC6A4/5HTT, PDLIM5, S100A10 and TNF mRNA expression in PBMCs collected at weeks 0, 2, 8, 29, 41, 43, 50, and 67. Patient received antidepressant treatment (Agomelatine, 25 mg/day) between week 29 and week 54. The reference genes used for normalization were: (i) RPL38 and RPLP0 to quantify S100A10 level of expression, (ii) ABL1 and MAPK1 for PDLIM5, (iii) SYNJ2 and ABL1 for IL8 and TNF, (iv) SYNJ2, SYP, and NGFR for CCL2, IL6, IL10 and SLC6A4/5HTT. The calibrator sample was sample at week 0. All qPCR experiments were conducted in duplicate and the mean Ct value was used for FC calculation.

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BMC Psychiatry

ISSN: 1471-244X

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