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Figure 3 | BMC Psychiatry

Figure 3

From: Investigation of G72 (DAOA) expression in the human brain

Figure 3

Validation of G72 primer sets using real-time RT-PCR. (A) Primer sets G72 #1, #2, #3, #4 and #5, which were designed within the most common G72 exons (see table 1 and Fig. 2B) were validated using human genomic DNA. Light grey: primer sequence within exon 4. Dark grey: primer sequence within exon 7. Hatched: primer sequence within exon 2. All primer sets gave a strong signal using human genomic DNA (A) and no signal using rat genomic DNA (not shown). (B) Primers sets within exon 2 (hatched) or exon 4 (light grey) readily detected a signal in cDNA that was isolated from HEK-293 cells expressing a His-tagged G72 cDNA, but not in mock-transfected cells (values within range of empty controls). The exon 7 reverse primer annealing sequence was not contained in our G72 cDNA construct and did thus not detect His-G72 (not shown).

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