Fig. 2

Immunohistochemical analysis of paraffin-embedded mouse brain specimens. a The cerebral cortex, basal nuclei, and cerebellum were widely stained by anti-GluN1 (NR1) antibody at a 200-fold dilution. b Particularly strong staining of the hippocampus and basal nuclei was observed with the anti-GluN2B (NR2B) antibody at a 200-fold dilution. The characteristic staining pattern was not observed with healthy control serum at a 1000-fold dilution (c) and with nonspecific IgG at a 200-fold dilution (d). e Representative images of extensive staining with anti-GluN1 from the serum of a 59-year-old male patient at onset at a 1000-fold dilution. f The characteristic stain was not observed with the use of serum from a healthy control patient whose symptoms improved at a 1000-fold dilution