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Table 1 PCR primers, PCR conditions and sequencing primers for genotyping five gene using pyrosequencing

From: Genetic association of human Corticotropin-Releasing Hormone Receptor 1 (CRHR1) with Internet gaming addiction in Korean male adolescents

  Primer for PCR Sequencing primer for Pyrosequencing Annealing
temperature
DRD4
VNTR
Forward 5’-GCGACTACGTGGTCTACTCG-3′ 60 °C
Reverse 5’-AGGACCCTCATGGCCTTG-3′
DAT1
VNTR
Forward 5’-TGTGGTGTAGGGAACGGCCTGAG-3′ 65 °C
Reverse 5’-CTTCCTGGAGGTCACGGCTCAAGG-3’
NET8
(rs5569)
Forward 5’-B-GTGAAGAGTTTCCGGTGTCGC-3’ 5’-GCATGGAGGCTGTCATC-3’ 57 °C
Reverse 5’-GAGGCATGGAGGCTGTCA-3’
CHRNA4
(rs1044396)
Forward 5’-CAGCCCTCTCCGTGCAAAT-3’ 5’-CTCTTCGGTGTCCCC-3’ 57 °C
Reverse 5’-B-GGTGCTGCGGGTCTTGAC-3’
CRHR1
(rs28364027)
Forward 5’-B-AGGAACCCTGGAGACAGAAGT-3’ 5′-GGTTAAGTGAGGGGAA-3’ 55 °C
Reverse 5′-TGGTATGGGGTGGTTAAGTGA-3’
  1. PCR polymerase chain reaction, B biotin
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