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Table 1 PCR primers, PCR conditions and sequencing primers for genotyping five gene using pyrosequencing

From: Genetic association of human Corticotropin-Releasing Hormone Receptor 1 (CRHR1) with Internet gaming addiction in Korean male adolescents

 

Primer for PCR

Sequencing primer for Pyrosequencing

Annealing

temperature

DRD4

VNTR

Forward

5’-GCGACTACGTGGTCTACTCG-3′

–

60 °C

Reverse

5’-AGGACCCTCATGGCCTTG-3′

DAT1

VNTR

Forward

5’-TGTGGTGTAGGGAACGGCCTGAG-3′

–

65 °C

Reverse

5’-CTTCCTGGAGGTCACGGCTCAAGG-3’

NET8

(rs5569)

Forward

5’-B-GTGAAGAGTTTCCGGTGTCGC-3’

5’-GCATGGAGGCTGTCATC-3’

57 °C

Reverse

5’-GAGGCATGGAGGCTGTCA-3’

CHRNA4

(rs1044396)

Forward

5’-CAGCCCTCTCCGTGCAAAT-3’

5’-CTCTTCGGTGTCCCC-3’

57 °C

Reverse

5’-B-GGTGCTGCGGGTCTTGAC-3’

CRHR1

(rs28364027)

Forward

5’-B-AGGAACCCTGGAGACAGAAGT-3’

5′-GGTTAAGTGAGGGGAA-3’

55 °C

Reverse

5′-TGGTATGGGGTGGTTAAGTGA-3’

  1. PCR polymerase chain reaction, B biotin