| Primer for PCR | Sequencing primer for Pyrosequencing | Annealing temperature | |
---|---|---|---|---|
DRD4 VNTR | Forward | 5’-GCGACTACGTGGTCTACTCG-3′ | – | 60 °C |
Reverse | 5’-AGGACCCTCATGGCCTTG-3′ | |||
DAT1 VNTR | Forward | 5’-TGTGGTGTAGGGAACGGCCTGAG-3′ | – | 65 °C |
Reverse | 5’-CTTCCTGGAGGTCACGGCTCAAGG-3’ | |||
NET8 (rs5569) | Forward | 5’-B-GTGAAGAGTTTCCGGTGTCGC-3’ | 5’-GCATGGAGGCTGTCATC-3’ | 57 °C |
Reverse | 5’-GAGGCATGGAGGCTGTCA-3’ | |||
CHRNA4 (rs1044396) | Forward | 5’-CAGCCCTCTCCGTGCAAAT-3’ | 5’-CTCTTCGGTGTCCCC-3’ | 57 °C |
Reverse | 5’-B-GGTGCTGCGGGTCTTGAC-3’ | |||
CRHR1 (rs28364027) | Forward | 5’-B-AGGAACCCTGGAGACAGAAGT-3’ | 5′-GGTTAAGTGAGGGGAA-3’ | 55 °C |
Reverse | 5′-TGGTATGGGGTGGTTAAGTGA-3’ |