Fig. 4

Effect of DCS injection on neural activity in schizophrenia-like model mice in vivo. a. Sample traces of Ca²⁺ responses before and after injection of DCS (i.p, 30 mg/kg) in either anti-Rag mice (A1) or anti-Nrg1 mice (A2) expressing CAG promoter virus. Scale bars, 0.2 min; 400% ΔF/F₀. b. Spontaneous Ca²⁺ responses were not altered by DCS injection, as measured by Ca²⁺ activity reported using CAG-GCaMP6s (B1), DCS-Synapsin (B2) or CaMKII-GCaMP6s (B3). Dotted line indicates baseline levels, normalized to 100%. c. No significant change in either mean spike rate (C1, anti-Rag, 88 units/3 mice; anti-Nrg1, 78 units/3 mice) or burst spike rate (C2, anti-Rag, 41 units/3 mice; anti-Nrg1, 55 units/3 mice)) after DCS injection in anti-Nrg1 mice. Dotted line indicates baseline levels, normalized to 100%