Table 2 A list of primers used for real-time PCR
Gene | Bi-directional primer sequence | Annealing temperature (°C) | Primer length (bp) |
|---|---|---|---|
β-actin (Reference) | Forward:5′CGAGTACAACCTTCTTGCAGC 3′ Reverse: 5′ ACCCATACCCACCATCACAC 3’ | 60 | 202 |
rno_circRNA_014900 | Forward:5′ CTTAGATGACCTGGAGAAGACCT 3′ Reverse: 5′ TGACTTGGTGCTGTTGACTTTAG 3’ | 60 | 124 |
rno_circRNA_013109 | Forward:5′ ATTATAGAGCTAATTACAACTTCCG 3′ Reverse:5′ TTATCTGAAGCATGTTAAGACAATA 3’ | 60 | 105 |
rno_circRNA_006565 | Forward:5′ CGACTTCAAAAGAGTTGTGGATT 3′ Reverse: 5′ TTCTCCTCGTGAGCTTTTTTCTC 3’ | 60 | 54 |
rno_circRNA_005442 | Forward:5′ ACCCCATGAGAAAGACCAGGTC 3′ Reverse:5′ CTGCTCTCTTCAAGTGAAAGACATC 3′ | 60 | 60 |
rno_circRNA_003460 | Forward:5′ CGCTAAGCATTTCTTTGGAA 3′ Reverse: 5′ GTAGTGGGTGTAGGGAGGAGA 3′ | 60 | 76 |