Fig. 2

Relative fluorescence intensities of the haplotype recombinants in SK-N-SH and HEK-293 cells. The haplotypes H2, H3, H4, H5, and H6 showed significantly different luciferase activity compared to the major haplotype H1 in SK-N-SH cell (a), the haplotype H2, H4 and H6 also showed significantly different luciferase activity compared to the major haplotype H1 in HEK-293 cell (b). ***p < 0.001; **p < 0.01, *p < 0.05. The relative fluorescence intensity of haplotype H1(with rs4140535T allele) was significantly higher than that of haplotype H3 (with rs4140535C allele) in the SK-N-SH cells, The relative fluorescence intensity of haplotype H2 (with rs1778258A allele) was much lower than that of haplotype H3 (with rs1778258G allele) in both cell lines, The haplotype H5 (with rs1228814A allele) appeared to upregulate the luciferase activity relative to haplotype H3 (with rs1228814C) in HEK-293 cells, the haplotype H6 (with rs17273700C-rs11568817G) had higher relative luciferase intensity than haplotype H5 (with rs17273700T-rs11568817T), and haplotype H4 (with rs130058T allele) had lower relative luciferase intensity than haplotype H6 (with rs130058A allele) in both SK-N-SH and HEK-293 cell lines (a,b)