H-group | Pat. No | CICs-Ext. (1:20) | pAG-Ext (1:2) | Ab-Ext (1:100) | Comment |
| 1 | 187 | 56 | 44 | |
| 2 | 280 | 70 | 157 | |
| 3 | 445 | 9 | 305 | |
| 4 | 179 | 25 | 157 | |
| 5 | 230 | 11 | 57 | |
| 6 | 1068 | 542 | 378 | |
| 7 | 678 | 104 | 135 | |
| 8 | 399 | 47 | 69 | |
| Mean ± SD | 433.25 ± 305.70 | 108 ± 178.22 | 185 ± 117 | |
L-group | Pat. No | CICs-Ext | pAG-Ext | Ab-Ext | Commenta |
| 9 | 66 | 34 | 79 | negative |
| 10 | 52 | 72 | 57 | negative |
| 11 | 154 | 7 | 84 | |
| 12 | 58 | 4 | 71 | negative |
| 13 | 137 | 45 | 181 | |
| 14 | 96 | 48 | 198 | |
| 15 | 164 | 51 | 60 | |
| 16 | 162 | 45 | 54 | |
| Mean ± SD | 111.13 ± 48.51 | 38.25 ± 22.85 | 90.5 ± 63.99 | |
H- + L-group | Mean ± SD | 272.2 ± 269 | 77.5 ± 131.1 | 137.7 ± 103.3 | |
- Abbreviations: Ext Extinction values at 405 nm, CICs BoDV-1-specific circulating immune complexes in blood plasma, specific for BoDV-1 antigens p40/p24, pAG antigens (p40/p24) in citrated blood plasma, Ab Antibodies. The values of CICs, pAG and Ab are given as extinction at 405 nm × 1000, measured by enzyme immune assays (EIAs) in the starting dilution in brackets for each test (see Methods)
- The cut off value of each test is ≥ 100. Results < 100 are regarded negative
- H-group = patients with high CICs; L-group = patients with low CICs
- aComment: Patients 9, 10 and 12 were negative at the time of initial sampling (week 0) shown here but developed CICs on week 2, lasting to week 6 up to week 16 (see follow-up data, Table 3)