Prevalence rate, predictors and long-term course of probable posttraumatic stress disorder after major trauma: a prospective cohort study

Background Among trauma patients relatively high prevalence rates of posttraumatic stress disorder (PTSD) have been found. To identify opportunities for prevention and early treatment, predictors and course of PTSD need to be investigated. Long-term follow-up studies of injury patients may help gain more insight into the course of PTSD and subgroups at risk for PTSD. The aim of our long-term prospective cohort study was to assess the prevalence rate and predictors, including pre-hospital trauma care (assistance of physician staffed Emergency Medical Services (EMS) at the scene of the accident), of probable PTSD in a sample of major trauma patients at one and two years after injury. The second aim was to assess the long-term course of probable PTSD following injury. Methods A prospective cohort study was conducted of 332 major trauma patients with an Injury Severity Score (ISS) of 16 or higher. We used data from the hospital trauma registry and self-assessment surveys that included the Impact of Event Scale (IES) to measure probable PTSD symptoms. An IES-score of 35 or higher was used as indication for the presence of probable PTSD. Results One year after injury measurements of 226 major trauma patients were obtained (response rate 68%). Of these patients 23% had an IES-score of 35 or higher, indicating probable PTSD. At two years after trauma the prevalence rate of probable PTSD was 20%. Female gender and co-morbid disease were strong predictors of probable PTSD one year following injury, whereas minor to moderate head injury and injury of the extremities (AIS less than 3) were strong predictors of this disorder at two year follow-up. Of the patients with probable PTSD at one year follow-up 79% had persistent PTSD symptoms a year later. Conclusions Up to two years after injury probable PTSD is highly prevalent in a population of patients with major trauma. The majority of patients suffered from prolonged effects of PTSD, underlining the importance of prevention, early detection, and treatment of injury-related PTSD.


Background
Major trauma, which can be defined as an injury with an Injury Severity Score (ISS) of 16 or higher, has a large impact, not in the least because of the relatively young age of the average severely injured patient [1]. A large proportion of the severely injured patients report significantly reduced health-related quality of life with functional limitations years after trauma [2][3][4]. In rehabilitation, the main focus lies in the treatment of physical injuries. Nonetheless, over the past decades the importance of psychological morbidity continued to gain attention, specifically concerning posttraumatic stress disorder (PTSD).
PTSD may result from any event that involves an injury, or threatened or actual death (of others). PTSD symptoms are characterized by re-experiencing, avoidance and hyper arousal, and may either appear immediately after the event or have a delayed onset [5]. In the general population PTSD prevalence rates between 2-4% have been found [6,7]. Trauma patients have relatively high prevalence rates of PTSD; prevalence rates up to 39% have been found one to four months after the injury [8]. At long-term follow-up (>1 year) PTSD prevalence rates vary from 5% among traffic injury victims [9] to 32% among major trauma patients [10].
Predictors of PTSD following major trauma are gender, age, presence of chronic illnesses, cause of injury, coping style, pain, cognitive functioning when discharged from the hospital and employment [11][12][13]. To our knowledge, the effect of pre-hospital trauma care (i.e. assistance of physician staffed Emergency Medical Services (EMS) at the scene of the accident) on the risk of developing PTSD has not yet been studied. Identifying subgroups at risk for PTSD is important for the targeting of PTSD prevention and to facilitate early treatment when PTSD has developed. Research has shown that PTSD can be effectively treated at an early stage [14]. However, symptoms of PTSD may not always develop immediately after the injury. In some cases, symptoms develop relatively long after sustaining the trauma. This time delay between the injury and PTSD may hamper identification of risk groups. Follow-up studies of injury patients may help gain more insight into the long-term course of PTSD and subgroups at risk for PTSD.

Aim of this study
The primary aim of our study was to assess the prevalence rate and predictors of probable PTSD in a sample of major trauma patients at one and two years after injury. In addition to the influence of socio-demographic, physical and injury related factors, we explored the association of pre-hospital trauma care, i.e., the presence versus absence of pre-hospital trauma care at the scene of the accident via assistance of physician staffed helicopter or other EMS teams. Secondly, this study aimed to assess the long-term course of probable PTSD following injury.

Study population and design
From January 2004 until July 2006, a prospective cohort study was conducted, including all consecutive major trauma patients with an Injury Severity Score (ISS) [15] of 16 or higher and aged 16 years or older, that were presented to a level I trauma center in a Dutch trauma region serving 4.9 million inhabitants. Patients that were pronounced Dead On Arrival were excluded. For the purpose of this study part of the data were derived from the Hospital Trauma Registry that documents the same variables as the Major Trauma Outcome Study database [16] (i.e., Age, Glasgow Coma Scale [17], Revised Trauma Score [18], Mechanism Of Injury, and injury specifics such as the Injury Severity Score (ISS)).
Missing data were obtained from the original ambulance charts. This PTSD study was part of a prospective cost effectiveness analysis of (helicopter) emergency medical services in the Netherlands [19]. One and two years after trauma all patients received a questionnaire. In absence of response patients received a phone call one month after the mailing in order to increase participation.
This study was conducted with the approval of the Ethics Committee Erasmus MC University Hospital and carried out in compliance with the Helsinki Declaration. All patients, or in case of pediatric patients, their parents of guardian, provided informed consent.

Impact of event scale
The impact of event scale (IES) may be used to assess symptoms of posttraumatic stress indicative of PTSD [20]. The IES is a self-report questionnaire that consists of 15 items, which measure intrusive re-experiences of the trauma and avoidance of trauma-related stimuli. The IES measures only two of the three main PTSD symptoms, namely intrusion and avoidance. By combining the 15 items the total IES-score, ranging from 0 through 75, can be calculated. Wohlfarth et al. showed that a cut-off score of 35 on the total IES-score produced sensitivity of .89, specificity of .94 when the DSM-IV was used as the diagnostic criteria for PTSD [21]. Therefore, we assumed an IES-score higher than 35 (IES ≥ 34) signifies symptoms of posttraumatic stress indicative of PTSD. Because the IES measures only two of the three main PTSD symptoms, the IES cannot be used to assess PTSD. Therefore, we indicate cases with an IES-score higher than 34 as cases with probable PTSD. The Dutch translation of the IES has been found to be valid and reliable [22].

Socio-demographic, injury, and health care related characteristics
From the literature, potential determinants of functional outcome were identified [23][24][25]. These determinants of functional outcome were grouped into sociodemographic (age and gender, education level, household composition, and pre-existing co-morbid illnesses), injury (ISS, Revised Trauma Score (RTS), and injury location), and health care related characteristics (HEMS (Helicopter Emergency Medical Services) or EMS). For the purpose of this study, several socio-demographic variables were grouped into two categories. Education was subdivided into primary school level or higher. Household composition was subdivided into households existing of a single person or more persons.
Co-morbidity is defined as the presence of any coexisting medical diseases or disease processes additional to the injury that the injury patients sustained [26]. The following diseases were assessed as co-morbid disease: asthma, chronic bronchitis, chronic non-specific lung disease; heart disease; diabetes; back hernia or chronic backache; osteoarthritis; rheumatoid arthritis and cancer.
Co-morbidity was subdivided into three groups. A comorbid condition was defined as a disease that existed at the time of trauma according to the patient or the family. Co-morbidity was categorised into a group without preexisting disease, a group with one co-morbid disease and thirdly, a group with two or more co-morbidities.
The injury diagnosis was verified at the individual level with information from the hospital discharge register according to the Abbreviated Injury Scale, 1990 Revision, update 1998 [27].

Pre-hospital trauma care
Pre-hospital trauma care was upgraded in the Netherlands in 1995, when physician staffed HEMS were introduced in addition to nurse staffed EMS. For all major trauma patients in this study it was registered which type of prehospital care (HEMS or EMS) was provided.

Statistical analysis
The statistical analyses were performed using the Statistical Package for the Social Sciences (SPSS) version 12.0 (SPSS, Chicago, IL, USA). We calculated the IES-score of each of the injury patients. The IES-score can only be calculated if all IES items are completed. In 4.5% of the cases data of one or two of the 15 IES items were missing. For these cases, the missing IES item was estimated by calculating the median value of 5 nearby points. The missing data was then imputed by the estimated values [28]. Fisher's exact tests were used to test for differences between the study population and respondents. Non-parametric variables (age, Glasgow coma Score, RTS, and ISS) were tested using the Mann-Whitney U-test.
Chi-square statistics (dichotomous variables) and Student t tests (continuous variables) were used to test for differences between injury patients with IES scores higher or lower than 35.
Univariate logistic regression analyses were used to determine the predictive value of socio-demographics, presence of co-morbid diseases, sustained injuries and pre-hospital trauma with regard to probable PTSD (IES ≥ 35).
Stepwise multiple regression analyses (enter method) was applied to investigate the association between demographics (block 1), hospitalization and comorbidity (block 2) posttraumatic stress symptoms indicative of PTSD (IES ≥ 35) (block 3). Variables with a p-value ≤ 0.1 in the univariate analysis were applied in the multivariate regression analysis.
Finally, we composed a flow chart including respondents that completed the IES at the two time points, to gain insight in probable PTSD courses.

Study population
During the study period of 30 months, 524 major trauma patients were admitted to the Emergency Department of the study hospital, of which 162 (31%) patients died within 30 days after hospital admission. Of the remaining 362 survivors, 332 were aged 16 years or older. These patients were included in the prospective cohort study on PTSD. One year follow-up measurements of 226 patients (response rate 68%) were obtained. Respondents were significantly older than non-responders (median age 40 versus median age 32, p < 0.05) and the proportion females was higher (non-responders: 18% female versus responders: 34% females, p < 0.05). Trauma mechanism, disturbance of vital parameters, severity of injury or type of pre-hospital trauma care did not differ significantly between responders and non-responders.
Two year follow-up measurements of 117 patients (response rate 52%) were obtained. A flow chart of the patient inclusion throughout the study is shown in Figure 1.
The median age of the respondents was 42 years and 66% were male (Table 1). Female respondents were significantly older than male respondents (median age 49 versus median age 40, p < 0.05).
The vast majority of patients (97%) sustained a blunt force trauma (non-penetrating trauma). The median ISS of the respondents was 22. The ISS did not differ significantly between female and male respondents (median ISS 20 versus median ISS 22). There were also no significant differences in trauma mechanism, disturbance of vital parameters or type of pre-hospital trauma care between male and female respondents.

Prevalence of probable PTSD
With reference to the 226 respondents that completed the one year follow-up questionnaire, 198 (88%) filled out the IES. At 2-years follow-up, 101 (86%) respondents filled out the IES. Table 2 shows the characteristics of the respondents an IES-score of 35 or higher, which indicates probable PTSD. At 1-year and 2-year follow-up 23% and 20% respectively of the respondents had probable PTSD.

Risk factors for developing PTSD
Univariate logistic regression analyses showed that comorbid disease and female sex were significantly associated with probable PTSD at one year follow-up. At two years follow-up injuries of the head and extremities were significantly associated with probable PTSD. Multivariate logistic regression analysis including socio-demographic, physical, injury and pre-hospital care variables, indicated that comorbid disease (OR 4.6; 95% CI, 2.0 to 10.6) and female gender (OR 2.4; 95% CI, 1.1 to 5.2) are strong independent predictors of probable PTSD one year after injury. Head injuries <3 (OR 0.1; 95% CI 0.02 to 0.66) and injuries to the extremities <3 (OR 0.2; 95% CI 0.03 to 0.99) were strong independent predictors of probable PTSD two years after injury.
Pre-hospital trauma care, i.e., assistance of physician staffed Emergency Medical Services (EMS) at the scene of the accident was not significantly associated with probable PTSD at either one or two years follow-up ( Table 3). Included in the prospective cohort study n=332

Course of PTSD
One year follow-up measurements n=226 Lost to follow-up n=106 Untraceable (97%) Unwillingness to participate (2%) Insufficient knowledge of Dutch or English language (1%) Two year follow-up measurements n=117 Lost to follow-up n=109 Figure 1 Flow chart of the patient inclusion throughout the study. criterion of PTSD later on. Of these five patients, four had an increase of more than 20 points on the IES scale. One patient had an almost similar IES score at the two time points (32 at 1-year follow-up versus 35 at 2-year follow-up).
Of the patients with symptoms indicative of probable PTSD at one year follow-up, 4 (21%) did not meet the PTSD criterion at two years follow-up. Three of these patients showed a decrease of more than 10 points on the IES scale. Approximately three in four patients (79%; n = 15) had persistent PTSD symptoms indicative of probable PTSD a year later.

Discussion
PTSD is common in a population of patients with major trauma. One year after trauma 23% of our sample had an IES-score of 35 or higher, indicating probable PTSD. At two years after trauma the prevalence rate of probable PTSD was 20%. Female gender and comorbid disease were strong predictors of probable PTSD one year following injury, whereas head injury and injury of the extremities <3 were strong independent predictors of this disorder at two year follow-up. Of the patients with probable PTSD at one year follow-up 79% had persistent PTSD symptoms a year later.
The prevalence rates that we found in our study are comparable to those found by Kreis et al. [29] and Soberg et al. [11], who reported prevalence rates of 23% and 19% respectively regarding trauma victims with severe injuries (ISS > 15). However, neither Kreis et al. [29] nor Soberg et al. [11] used the IES to assess PTSD, which may have affected the PTSD prevalence rates that were found.
Holbrook et al. [10] did use the IES to assess prevalence of probable PTSD regarding a sample of trauma patients, yet they found a higher prevalence rate of 32% at 18 months follow-up. This difference in prevalence rate might be explained by differences in patient populations.
A second explanation for the differences in prevalence rates of probable PTSD may be the IES cut-off point that was used. Holbrook et al. [10] used an IES-score greater than 24 to identify patients with probable PTSD, whereas in the current study a cut off of 35 was used. When we applied a similar IES cut-off point of 24, the prevalence rate of probable PTSD one year following trauma increased to 36%. At two years follow-up the prevalence rate of probable PTSD increased to 26%. These prevalence rates are in the same range as reported by Holbrook et al. Evidence suggests it is important to use a high IES cut-off score that incurs a high specificity to avoid over diagnosing of PTSD in a comprehensive population with a relative low PTSD prevalence [21,30].
The existence of PTSD symptoms was measured with the IES rather than Clinician-Administered PTSD Scale  for DSM-IV (CAPS). Important to note is that the IES is a self-report questionnaire that measures only two of the three main PTSD symptoms, namely intrusion and avoidance. Hyperarousal, the third main PTSD symptom, is not measured by the IES. The IES is not a diagnostic tool, i.e., it is not designed to diagnose mental disorders according to the DSM-IV (the fourth edition of the diagnostic and statistical manual for psychiatric disorders). Consequently, cases that in the current study were identified as having PTSD symptoms might not meet the DSM-IV criteria of clinical PTSD, and inversely. The use of different diagnostic instruments may be a methodological reason for differences in prevalence rates between studies. Presumably, the fact that in this study the IES has been used, and consequently PTSD symptoms excluding hyperarousal symptoms have been assessed may have resulted in a relatively high rate of probable PTSD. In this study, a quarter of the sample met criteria for a moderate TBI, and another 20% met criteria for a severe Table 3 Odds ratios a (OR) and 95% confidence interval (CI) for the association of probable PTSD (IES-score ≥ 35) with characteristics of the respondent/injury  TBI. TBI can have serious effects on communication and cognition, and the presence of severe TBI will impact upon any assessment of PTSD. Moreover, a large prospective cohort investigation of injured trauma survivors with TBI in the United States found an elevated risk of PTSD among patients with mild TBI as more than one in five (22%) was diagnosed with PTSD at one year follow up [31].
Our study focused on a single stressor, i.e., injury, whereas PTSD generally originates from cumulative exposure to traumatic stressors. The presence of traumatic stressors also influences the probability of spontaneous remission from PTSD [32,33]. This means that the level of other traumatic stressors may affect to a large extent the prevalence rates that were found. At long-term follow-up (>1 year) PTSD prevalence rates from 5% [9] to 32% [10] have been reported regarding trauma patients. This variety in PTSD prevalence rates can therefore be explained by differences in exposure to traumatic stressors other than injury.
To identify subgroups at risk for long-term probable PTSD, risk factors for the development of probable PTSD one and two year following injury were assessed. The results of our study indicate a strong association between female gender and probable PTSD. This association is in line with findings in the general literature [34] and has been reported by other studies on PTSD following injuries [10,[35][36][37]. In literature several explanations were found for these gender differences in PTSD risk, such as women's stronger perceptions of threat and loss of control and higher levels of peritraumatic dissociation, as well as gender-specific acute psychobiological reactions to trauma [34]. Another independent predictor of probable PTSD at one year follow-up was the presence of one or more co-morbid diseases. Severity of the injury, reflected by the ISS, was not significantly associated with probable PTSD. This is in accordance with findings from previous studies [11,12,29,38]. However, a limitation of our study is that the comorbidity measure is very simple, since it groups comorbidities into none, one or more than one. No distinction was made whether the comorbidities are related to pre-existing psychiatric disorders and/or substance abuse. If this distinction was made, it would shed more light on the relation between different types of comorbidity and PTSD.
Furthermore, peritraumatic processing, social support, peritraumatic dissociation or other predictors of PTSD were not included in this study [39,40].
Pre-hospital trauma care, i.e., the presence of helicopter or other physician staffed EMS teams at the scene of the accident was not significantly associated with probable PTSD at either one or two years follow-up. Dispatch is based on the apparent seriousness of a distress call or trauma mechanism, or based on patients' condition as assessed by ambulance personnel at the scene of the accident. In other words, helicopter or other physician staffed EMS teams attend to the most severe cases and specific trauma mechanism. Our hypothesis was that cases with pre-hospital trauma care at the scene may be more prone to develop PTSD. The finding that pre-hospital care was not significantly associated with PTSD may be explained by the finding that trauma mechanism, disturbance of vital parameters or severity of injury are not significantly associated with probable PTSD.
The results of this study may not be generalizable to young male major trauma patients, because respondents who were willing to participate in the study were significantly older and significantly more likely to be female.
A strength of this study was that it did not focus solely on prevalence rates of probable PTSD, but also addressed the course of probable PTSD at long-term follow-up. Previous research on the course of PTSD revealed that patients experience symptoms for one year or longer [41,42]. The flowchart depicting injury patients with and without symptoms at one and two years following injury allowed us to gain insight into the development of probable PTSD over time in a sample of severely injured trauma patients. The flowchart showed that PTSD symptoms fluctuate over time; patients meet criteria or cut-off at some time points, and at other points they fell just below, but that the majority of patients with probable PTSD at one year follow-up still meet the IES criterion for PTSD at two years follow-up. This indicates that many patients might suffer from prolonged effects of PTSD.
Most likely PTSD symptoms occur during the first year after the accident, rather than between year one and two after the injury. However, in the current study baseline information on PTSD symptoms was not gathered. As a result, the course of probable PTSD in the first year after the injury could not be analyzed, nor does this study provide information about pre-existing PTSD.

Conclusions
We conclude that almost one in four major trauma patients have an IES-score of 35 or higher, indicating evident symptoms of PTSD one year after sustaining the injuries. At two years follow-up almost one in five major trauma patients suffered from probable PTSD. Female gender and co-morbidity were the strongest independent predictors of this disorder. Research on the course of PTSD symptoms showed that major trauma patients can develop and recover from probable PTSD at different time points, but the majority of patients with probable PTSD at one year follow-up still met the IES criterion for probable PTSD at two years follow-up. Since PTSD raises a major and often prolonged barrier for full recovery of injury patients, the development and evaluation of ED and hospital-based policies for early diagnosis and treatment of PTSD should be stimulated.