Figure 5

Attempted detection of G72 protein in human samples by western blotting. Western blot membranes were probed with anti-G72 #1410 and #1411 as indicated above and visualised using the LI-COR Odyssey^® Infrared Imaging System. Lane 1: total protein lysate from His-G72-transfected HEK-293 cells (positive control). 2: no sample. 3–8: Clontech/BD Biosciences human "protein medleys"; 3: spinal cord. 4: testis. 5: amygdala. 6: cerebellum. 7: frontal lobe. 8: fetal brain. 9: total protein lysate from rat cortex, postnatal day 14 (negative control – no G72 gene in rat). M: molecular weight standards. No specific signal at the expected size of ~18 kDa was detected in any of the human samples. A very weak signal at ~15 kDa was seen in testis, cerebellum and fetal brain (lanes 4, 6, 8). Anti-G72 #1411 detected a strong unspecific signal of unknown identity in all human CNS samples (lanes 3, 5, 6, 7, 8). Blots were reprobed with anti-actin as a loading control (lower panel).