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Table 1 Primer and probe sequences for G72 and cyclophilin

From: Investigation of G72 (DAOA) expression in the human brain

Primer name & location Primer and probe sequence (F: forward, R: reverse, P: probe)
G72 #1 (Exon 4) F: 5'-GGATGGAAGAGAAGGCATGAGG-3'
  R: 5'-TCTGCATAGGGCTGAGGAAGG-3'
  P: 5'-ATTTGGAAATGGCACAGAGG-3'
G72 #2 (Exon 7) F: 5'-GCTGAATGAGTTTGGAAGCA-3'
  R: 5'-TGGGTCCCAGACACAGAGT-3'
G72 #3 (Exon 2) F: 5'-CATTGGGTAAAATCTACTTCATAGG-3'
  R: 5'-GAGTTTAGAGAGTTTTCAGATTTGC-3'
G72 #4 (Exon 4) F: 5'-GCATGAGGACGGCTATTTGG-3'
  R: 5'-TCTGCATAGGGCTGAGGAAGG-3'
G72 #5 (Exon 7) F: 5'-CCGCAGGCAGCCTCTAGAAC-3'
  R: 5'-TCAGAAGGATTGGCTGGGAAG-3'
  P: 5'-CCAGCAAAAAGACCAGTCATGCAACCA-3'
Cyclophilin F: 5'-TGAGACAGCAGATAGAGCCAAGC-3'
  R: 5'-TCCCTGCCAATTTGACATCTTC-3'
  P: 5'-AGCACCAATATTCAGTACACAGCTTAAAGCTATAGGTT-3'
  1. Primers and probes for G72 Real-Time RT-PCR (SYBR® Green and TaqMan®) and for the housekeeper gene cyclophilin are shown above. For exon numbering, compare Fig. 2B.