Fig. 3

Elevated basal neuronal activity in mice injected with anti-Nrg1 antibodies. a. Neural activity measured using CAG-GCaMP6s virus in FrA neurons. (A1) Sample images of neurons expressing CAG-GCaMP6s virus in the FrA, in mice injected with either anti-Rag antibody (left, Rag) or anti-Nrg1 antibody (right, Nrg1). (A2) Individual spontaneous Ca²⁺ response showed increase in amplitude (amplitude of ΔF/F), and frequency (number of events per min), in mice injected with anti-Nrg1 antibodies. (A3) Total Ca²⁺ activity (integrated area of all events within 1 min) was also elevated in anti-Nrg1 mice. Scale bars, 10 μm. b. Neuronal activity in the excitatory neurons measured using CaMKII-GCaMP6s virus in the FrA. (B1) Sample images of neurons expressing CaMKII-GCaMP6s virus in the anti-Rag (left) and anti-Nrg1 mice (right). (B2) Amplitude and frequency of individual spontaneous Ca²⁺ responses were significant higher in the anti-Nrg1 mice, compared to the anti-Rag mice. (B3) Total Ca²⁺ activity level was higher in the anti-Nrg1 mice compared to the anti-Rag control mice. Scale bars, 10 μm. c. In vivo single unit recording. (C1) Sample recording in an anti-Rag (upper) or anti-Nrg1 mouse (lower). Each vertical line represents a single spike. Scale bar, 50 s. (C2) Significantly higher mean spike rate was seen in the anti-Nrg1 mice, compared to the anti-Rag mice. (C3) Burst spike rate was not significantly different between the anti-Nrg1 and anti-Rag mice. *, P < 0.05; **, P < 0.01, ***, P < 0.001