Table 1 Primer sequence and PCR product length of housekeeping gene and target genes
Gene | Primer types | Primer sequence | Tm value(°C) | PCR product length (bp) |
|---|---|---|---|---|
GAPDH | Forward | GGCCTCCAAGGAGTAAGACC | 60.07 | 122 |
Reverse | AGGGGAGATTCAGTGTGGTG | 59.96 | ||
MTOR | Forward | AGCCGGAATGAGGAAACC | 60.01 | 232 |
Reverse | CAAATCTGCCAATTCGGG | 60.00 | ||
DEPTOR | Forward | ATTGTTGGTGACGCGGTT | 59.97 | 137 |
Reverse | AGCCCGTTGACAGAGACG | 59.98 | ||
RICTOR | Forward | AAGGCCAAACAGCTCACG | 59.98 | 230 |
Reverse | ACTCCATGAGGGTGGCAA | 60.05 | ||
RAPTOR | Forward | CAGGACTTGCTGGTGGCT | 59.98 | 84 |
Reverse | GCTGACGGGAGTGCAGTT | 59.99 |