Fig. 5

Effects of minocycline and antipsychotics on inflammatory cytokines production of microglia activated by LPS. The cells were randomly divided into 5 groups. The blank control group was treated with nothing, the LPS group was treated with 1.0 ug/ml LPS for 24 h. The three drug groups were pretreated with 10 μmol/L minocycline, 10 μmol/L risperidone and 50 μmol/L haloperidol respectively for 24 h, and then 1.0 ug/ml LPS was added for 24 h. Then, the conditioned medium was collected and the production of IL-6 (A), IL-1β (B), and TNF-α (C) was quantified by ELISA kit. The results were from six independent experiments. ^###p < 0.001 was compared with the blank control group; **p < 0.01 and ***p < 0.001 were compared with the LPS group.