Fig. 6

Effects of minocycline and antipsychotics on NO and iNOS expression of microglia activated by LPS. The cells were randomly divided into 5 groups. The blank control group was treated with nothing, the LPS group was treated with 1.0 ug/ml LPS for 24 h. The three drug groups were pretreated with 10 μmol/L minocycline, 10 μmol/L risperidone and 50 μmol/L haloperidol respectively for 24 h, and then 1.0 ug/ml LPS was added for 24 h. The production of NO (A) in conditioned medium was measured by Griess’s reagent assay, and the band density of iNOS (B) was used to quantify the expression level normalized with a band density of α-Tubulin. The expression of iNOS was determined by western blot analysis (C). ^###p < 0.001 was compared with the blank control group; **p < 0.01 and ***p < 0.001 were compared with the LPS group. The original image of the full gels/blots was included in the Supplementary Fig. S1.