Fig. 7

Effects of minocycline and antipsychotics on MAPKs inflammatory signaling pathway in BV-2 microglial cells. The cells were randomly divided into 5 groups. The blank control group was treated with nothing, the LPS group was treated with 1.0 ug/ml LPS for 24 h. The three drug groups were pretreated with 10 μmol/L minocycline, 10 μmol/L risperidone and 50 μmol/L haloperidol respectively for 24 h, and then 1.0 ug/ml LPS was added for 24 h. The band density was used to quantify the expression levels of JNK, ERK, and pho-p38/ p38 normalized with a band density of α-Tubulin (A). The expression of the proteins was determined by western blot analysis (B). ^##p < 0.01 and ^###p < 0.001 were compared with the blank control group; *p < 0.05, **p < 0.01 and ***p < 0.001 were compared with the LPS group. The original image of the full gels/blots was included in the Supplementary Fig. S2, Fig.S3, Fig.S4, and Fig.S5.